Effect of adding Polyethylene Glycol and Polyvinyl Pyrrolidon on In Vitro Gas production of Pomegranate seed

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The aim of this study was to determine the chemical composition including tannin content of pomegranate seed supplemented with different levels of tannin-binding agents (polyethylene glycol (PEG) and polyvinylpyrrolidone (PVP)) and gas production characteristics using in vitro gas production technique. The treatments were PS (control), PS+150 mg PEG, PS+300 mg PEG, PS+150 mg PVP and PS+300 mg PVP. At the 48 h incubation times, the gas production volume of PS, PS+150 mg PEG, PS+300 mg PEG, PS+150 mg PVP and PS+300 mg PVP were 109.47, 129.71, 125.79, 125.22 and 99.99 ml/g DM, respectively, and treatments PS+150 mg PEG, PS+300 mg PEG and gas production of PS+150 mg PVP treatment was higher (P<0.05) than other treatments. This value for treatment of PS+300 mg PVP revealed to be lower than the others (P<0.05). Addition of PEG and PVP could overcome adverse effects of tannins on nutrient availability as indicated by gas production parameters. Addition of PEG and PVP (except 300 mg PVP level) inactivated effects of tannins and increased gas production in pomegranate seed.

Key Words: By-Product, In Vitro Gas Production, Polyethylene Glycol, Polyvinylpyrrolidon, Pomegranate Seed, Tannin.

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