Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese

Özlem TURGAY, Sowayla MIKAEEL
1.709 462

Öz


Bu çalışmada Duhok peynirinde of E. coli O157:H7'nin varlığı kültür ve Polimeraz Zincir Reaksiyonutekniği (PZR) ile araştırılmıştır. Peynir örnekleri (n:120) Zakho, Akre, Amedy ve Duhok şehirlerinden Eylül 2011Şubat 2012 tarihleri arasında yerel market ve bakkallardan temin edilmiştir.X-gal pozitif, MUG negatif, indol pozitif olan %10 (12/120) oranında şüpheli koloni tespit edilmiştir. Şüphelikolonilere biyokimyasal testler uygulanmış (API E 20 ve latex aglütinasyon testi) ve 7 (%5,83) örnekte E. coliO157:H7 tespit edilmiştir. Bu izolatlararfbO157 ve flicH7 primerleri kullanılarak PCR yöntemi ile doğrulama işlemiuygulanmıştır. İzolatlar %3,33 oranında O157 ve 0,83 oranında H7 antijeni pozitif olarak tespit edilmiştir. PCRanalizlerinin sonunda örneklerin %0,83'ünün E. coli O157:H7 olduğu gözlenmiştir

Anahtar kelimeler


Duhok Peyniri, Escherichia coli O157:H7, Kültür tekniği, PZR tekniği

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DOI: http://dx.doi.org/10.18016/ksujns.94848

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