The Effect of Some Sugars on the Growth of Aspergillus niger
Abstract: The majority of black Aspergilli, including Aspergillus niger. Here, we provide to evidence that the exploration of sugar uptake in the filamentous fungus A. niger as a sole carbon source. The goal of this research line is determination of the growth of fungi was evaluated every 24h, measuring the colony diameter (cm). A. niger was inoculated onto two culture media: PDA: for maintains a strain as pure while Czapeck Dox Agar was used in investigation into their carbon requirement, using five different carbon sources (vizs. glucose, fructose, sucrose, maltose, and starch). The fungus was tested grew sparsely on the basal medium lacking in carbon, which was the control. However this fungus was found to vary from their ability to use the supplied sources of carbon. Fructose and sucrose were found to be suitable sources of carbon for a fungal isolates, whereas glucose and maltose proved good carbon source to have a higher affinity. Starch as a polysaccharide, was a poor source of carbon for the growth of this isolate. Despite earlier claims, saccharides rather than monosaccharide were breakdowns extracellularly by means of a broad range of extracellular enzyme activities from Aspergillus niger.
Key words: Aspergillus niger, Saccharides utilization, fungal growth
Tam metin:PDF (English)
Adejuwon, A. O., Olanike, O., Olabisi, A., 2012. Production of amylase from Aspergillus niger using defined synthetic growth medium and also rice (Oryza sativa) as growth substrate. E3 Journal of Medical Research, 1 (7): 091–094.
Akroum, S., 2014. Aspergillus niger growth on Polyphenolic carbon source and optimization of the tannase production. G.J.B.B., 3 (3): 246–249.
Bedan, D. S., Aziz, G. M., Al - Sa’ady, A.J.R., 2014. Optimum conditions for α- amylase production by Aspergillus niger mutant isolate using solid state fermentation. Current Research in Microbiology and Biotechnology, 2 (4): 450-456.
Batista, L. R., Silval, D. M., Rezende, E. F., Fungaro, M. P., Alves, E., Sartori, D., 2011. Identification of fungi of the genus Aspergillus section niger using polyphasic Microbiology, 42: 761-773. Brazilian Journal of
Eaton, A. D., Clesceri, L. S., Greenberg, A. E., 1998. Standard Methods for the Examination of Water and Waste water, 20th Ed., American Public Health Association. Washington, USA.
Fang, L., Wenqing, L., Darin, R., Tingyue, G., Murray, M., 1998. Inhibition of extracellular protease secretion by Aspergillus Biotechnology Letters, 20 (6): 539–542. cell immobilization.
Management, 15 (1): 203–206.
Lamb, R. J., 1974. Effect of D-glucose on utilization of single carbon sources by ectomycorrhizal fungi. Trans. Br. Mycol. Soc., 63 (2): 295-306.
Maharshi, A. R., Thaker, V. S., 2012. Growth and Development of Plant Pathogenic Fungi in Define Media. European Journal of Experimental Biology, 2 (1): 44-54.
Mehrotra, B. S., Kumar D., 1961. Study on Penicillia. University of Allahabad, 28 (1): 41-48.
Mchunu, N. P., Permau, K., Alam, M., Singh, S., 2013. Carbon utilization profile of a thermophilic fungus, Thermomyces microarray. Biotechnology, 4: 24-32. using phenotypic Advances in Bioscience and
Mcnaught, A. D., 1996. Nomenclature of Carbohydrate. The Royal Society of Chemistry. Thomas Graham House. Science Park. Milton Road. Cambridge. UK.
Omemu, A. M., Akpan, I., Bankole, M. O., Teniola, O. D., 2005. Hydrolysis of raw tuber starches by amylase of Aspergillus niger AM07 isolated from the soil. Afr. J. Biotechnol., 4 (1): 19-25.
Pandey, A., Nigamp, V. T., Socco, L., Singh, D., Mohan, R., 2006. Advances in microbial amylases. Biochem, 31: 35-152
Peña, M. S., 2010. Systems Biology of Glucose Sensing and Repression in Aspergillus niger. PhD thesis of Chalmers University. Goteborg. Sweden.
Salzer, P., Hager, A., 1991. Sucrose utilization of the ectomycorrhizal Hebeloma crustuliniforme depends on the cell wall- bound invertase activity of their host Picea abies. Bot. Acta, 104: 439-445. Amanita muscaria and
Samson, R. A., Hong, S., Frisvad, J. C., 2006. Old and new concepts of species differentiation in Aspergilli. Med. Mycology, 44 (1):133–148.
Sati, S.C., Bisht, S., 2006. Utilization of various carbon sources for the growth of waterborne conidial fungi. The Mycological Society of America, 98 (5): 678– 681.
Sasi, A., Kani, M., Panneerselvam, A., Jegadeesh, G., Muthu, K., Kumar, M.R. 2010. Optimizing the conditions of amylase by an Esturian strain of Aspergillus spp. Afr. J. Microbiol. Res., 4 (8): 581- 586.
Schrickx, J. M., Krave, A. S., Verioes, J. C., Van Den Hondel, Verseveld, H. W. 1993. Growth and product formation in chemostat and recycling cultures by Aspergillus niger N402 and a glucoamylase overproducing transformant, provided with multiple copies of the glaA-gene. Journal of General Microbiology, 139: 2801–2810. A. H., Van
Snellman, C. A., Greathouse, J. E., 1996. The Effect of Glucose on the Growth of Filamentous Fungi in Jet Fuel. Dean of the Faculty. USAF Academy Colorado. USA.
Schuster, E., Dunn-Coleman, N., Frisvad, J., Van Dijck, P., 2002. On the safety of Aspergillus niger – a review. Appl. Microbial Biotechnology, 59: 426-435.
Tandon, R. N., Bilgrami, K. S., 1956. Assimilation of disaccharides by some fungi causing “Leaf Spot” disease, pp. 274–284.
Thyagarajan, R., Raja, S. K., 2010. Influence of carbon source on phytase production by Aspergillus niger. International Journal of Biological Technology, 1 (2): 0976–4313.
Trinci, A.P.J., 1969. A Kinetic Study of the Growth of Aspergillus nidulans and Other Fungi. Journal Gen. Microbial, 57: 11–24.
Walsh, J. H., Harley, J. L., 1962. Sugar absorption by Chaetomium globosum. Department of Agriculture, pp. 299–313.
Yuan, X. L., Van der Kaaij, R. M., Van der Hondel, C., Punt, P. J., Van der Maarel, M., 2008. Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles. Mol. Genet. Genomics, 279: 545- 561.