Effect of Watery and Alcoholic Medicinal Plants Extractions on In-Vitro Ruminant

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Abstract: Two methods of extraction of some medicinal plants, water and alcoholic extractions for Nigella sativa, rosemary, borage, lemon balm and licorice root (Glycyrrhiza glabra) were employed to test their effects on in-vitro rumen digestion trial. Total phenolics contents significantly increased in their water extractions in contrast to their alcoholic extractions. Dry matter digestibility (DMD) significantly increased the water extractions of lemon balm, rosemary and licorice root: 68.47, 70.16 and 73.49 % compared to the control with 76.92%, respectively. Similar trend was observed with organic matter digestibility values (OMD): 69.71, 72.93 and 74.11% compared to the control with 77.51%, respectively. However, there was an overall significant increase in the studied digestion coefficient values with alcoholic extractions of the same plants. In conclusion, the alcoholic extractions of some medicinal plants can be recommended to be used in ruminant nutrition when these medicinal plant extracts are to be included in their feed for better digestibility values of dry matter and organic matter.

Key Words: Medicinal plants, in vitro, digestibility

Anahtar kelimeler

Medicinal plants, in vitro, digestibility

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PDF (English)

DOI: http://dx.doi.org/10.18016/ksujns.90521


A.O.A.C. 2005. Association of Official Analytical Chemists, Official Methods of Analysis, 19thedn. Washington, DC.

Al-Sarraj, S.M., Redha, F.M.J., Mahmoud, M.J., Hussein,W.A. 1985. Modiced extraction procedure for the active constituents of some Iraqi medicinal plants. Fitoterapia LVI:56-58.

Duncan, D.B. 1955. Multiple ranges and multiple "F" test. Biometrics, 11: 1-12. Folin, O., Denis, W.

19 Phosphotungstic

phosphomolybdic compound as color reagents. J.

Biol. Chem. 12: 239-243.

Hassan, S.A., K.M. Hassan. 2010. The effect of supplementation of medicinal plants and probiotic agent on growth rate and some blood components of karadi lambs. Egyptian J. Applied Sciences, 25(8A):408-419.

Hassan, S.A., Hassan, K.M., Al-Rubeii, A. 2011. Carcass yield and characteristics of Karadi lambs as affected by dietary supplement of rumen under gradable nitrogen fed with Nigella sativa. African J. of Biotechnology, 10(8):1491-1495.

Hassan, S.A., Tawffek, J.A. 2009. Effect of washing and physical form of chemically treated barley straw on nutritive value, phenolic compound and activity of rumen bacteria. 1-Sodium hydroxide treatment. The Iraqi J. of Agricultural Sciences, 40 (1):138-147.

Joslyn, M.A. 1950. Methods in Food Analysis. Applied to plant products. Academic Press Inc. New York. Mhamdi, B.,

Aidi Wannes, W., Sriti, J., Jellali, I.,

Ksouri, R., Marzouk, B. 2010. Effect of harvesting

time on phenolic compounds and antiradical

scavenging activity of Borago officinalis seed

extracts. Industrial Crops and Products, 31(1): 1-4.

Nergiz, C., Otles, S. 1993. Chemical composition of Nigella sativa L. seeds. Food Chem., 38:259.

Rababah, T.M., Hettiarachchy, N,S., Horax, R. 2004. Total phenolics and antioxidant activities of fenugreek, green tea, black tea, grape seed, ginger, rosemary, gotu kola, and ginkgo extracts, vitamin E, and tert-butylhydroquinone. J. Agric. Food Chem., 52 (16):5183–5186.

SAS, 2001. SAS/STAT User’s Guide for Personal Computers. Release 6.12. SAS Institute Inc., Cary, NC, USA, p.34-55.

Sunita, M., Dhananjay, S. 2010. Quantitative analysis of total phenolic content in Adhatoda vasica nees extracts. International J. Pharm. Tech. Res., 2(4): 2403-2406.

Tanja, R., Barbara, J. 2009. Antimicrobial activity of rosemary extracts (Rosmarinus officinalis L.) against different species of Listeria. Acta Agricultura Slovenica, 93(1): 51 – 58.

Tawffek, J.A., Hassan, S.A. 2014. Nutrition Science. Al-Dar Aljamaeh Press Inc. Baghdad, Iraq.

Tilley, J.M., Terry, R.A. 1963. A two stage technique for in vitro digestion of forage crops. J. Br. Grassland Sci., 18: 104-111.

Zanouny, A.I., Abd Elmoty, A.K.I., El- Barody, M.A.A., Sallam, M.T., Abd Elhakeam, A.A. 2013. Effect of Nigella sativa seeds supplementation on nutritive values and growth performance of Ossimi sheep. Egyptian J. of Sheep & Goat Sci., 8 (1):47- 56.