Stepwise purification of Phenylalanine ammonia lyase (PAL) enzyme obtained from lucerne (Medicago sativa cv. Vertus) inoculated with Verticillium dahliae

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ABSTRACT: An increased activity in phenylalanine ammonia lyase (EC, PAL) enzyme in lucerne seedlings (Medicago sativa L. cv. Vertus) following inoculation with Verticillium dahliae was measured after 48 h of inoculation.  Purifications of PAL with 0-50% (NH4)2SO4 precipitation and Sephadex G-100 gel filtration resulted in 1.75 and 1.97 fold increases with 75 and 60 % recoveries, respectively.  This study showed that the purification of the enzyme clearly resulted in significant differences in respect to activities between fractions.  This property could be important for further characterization of pathogen-related proteins.

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