Stepwise purification of Phenylalanine ammonia lyase (PAL) enzyme obtained from lucerne (Medicago sativa cv. Vertus) inoculated with Verticillium dahliae
ABSTRACT: An increased activity in phenylalanine ammonia lyase (EC 18.104.22.168, PAL) enzyme in lucerne seedlings (Medicago sativa L. cv. Vertus) following inoculation with Verticillium dahliae was measured after 48 h of inoculation. Purifications of PAL with 0-50% (NH4)2SO4 precipitation and Sephadex G-100 gel filtration resulted in 1.75 and 1.97 fold increases with 75 and 60 % recoveries, respectively. This study showed that the purification of the enzyme clearly resulted in significant differences in respect to activities between fractions. This property could be important for further characterization of pathogen-related proteins.